A role for cell-cycle-regulated histone H3 lysine 56 acetylation in the DNA damage response. Chromatin- and transcription-related factors repress transcription from within coding regions throughout the Saccharomyces cerevisiae genome. Processing mechanism and substrate selectivity of the core NuA4 histone acetyltransferase complex. Asf1 can promote trimethylation of H3 K36 by Set2. A role for Gcn5 in replication-coupled nucleosome assembly. Nucleosome assembly by a complex of CAF-1 and acetylated histones H3/H4. Chaperone control of the activity and specificity of the histone H3 acetyltransferase Rtt109. Dimethylation of H3K4 by Set1 recruits the Set3 histone deacetylase complex to 5′ transcribed regions. DSIF and RNA polymerase II CTD phosphorylation coordinate the recruitment of Rpd3S to actively transcribed genes. Histone H3–K56 acetylation is catalyzed by histone chaperone-dependent complexes. Cell cycle- and chaperone-mediated regulation of H3K56ac incorporation in yeast. Genome-wide replication-independent histone H3 exchange occurs predominantly at promoters and implicates H3 K56 acetylation and Asf1. Dynamics of replication-independent histone turnover in budding yeast. Evidence for eviction and rapid deposition of histones upon transcriptional elongation by RNA polymerase II. Evidence for distinct mechanisms facilitating transcript elongation through chromatin in vivo. Splitting of H3–H4 tetramers at transcriptionally active genes undergoing dynamic histone exchange. The histone variant H3.3 marks active chromatin by replication-independent nucleosome assembly. Histone H3.1 and H3.3 complexes mediate nucleosome assembly pathways dependent or independent of DNA synthesis. Genome-scale profiling of histone H3.3 replacement patterns. Nucleosome displacement in transcription. Infrequently transcribed long genes depend on the Set2/Rpd3S pathway for accurate transcription. Phosphorylated Pol II CTD recruits multiple HDACs, including Rpd3C(S), for methylation-dependent deacetylation of ORF nucleosomes. Histone H3 methylation by Set2 directs deacetylation of coding regions by Rpd3S to suppress spurious intragenic transcription. By suppressing spurious cryptic transcripts from initiating within ORFs, this pathway is essential to maintain the accuracy of transcription by RNA polymerase II.Ĭarrozza, M. Thus, Set2 functions both to suppress the incorporation of acetylated histones and to signal for the deacetylation of these histones in transcribed genes. In addition to its function of targeting and activating the Rpd3S complex, H3K36 methylation suppresses the interaction of H3 with histone chaperones, histone exchange over coding regions and the incorporation of new acetylated histones. Here we show that, in yeast, co-transcriptional acetylation is achieved in part by histone exchange over ORFs. Although the co-transcriptional deacetylation pathway is well characterized, the factors responsible for acetylation are as yet unknown. In the absence of Set2, H3K36 or Rpd3S acetylated histones accumulate on open reading frames (ORFs), leading to transcription initiation from cryptic promoters within ORFs 1, 3. The recommended size for a header display image is 1200 pixels wide (height to maintain aspect ratio).Set2-mediated methylation of histone H3 at Lys 36 (H3K36me) is a co-transcriptional event that is necessary for the activation of the Rpd3S histone deacetylase complex, thereby maintaining the coding region of genes in a hypoacetylated state 1, 2. This image size would be absolutely fine for the photo gallery, but will look slightly poor and perhaps pixelated uploaded as a header image display. So in this particular instance the photo is 425 pixels: Then to see what pixel size it is click into 'Details' and scroll down until you see 'Image' and then 'Dimensions'. This allows you to see the size of the photo file. If you're unsure of the image quality, a good check is to right click on the image where it is saved on your computer, then click into 'Properties' and the following pop-up will appear on your screen: You do not crop in too much to your image which will distort the pixelation.You are uploading the original photo to your website, not an image copied and saved from another website or social media channel. Your photo has been taken with a good camera and is of a high resolution.To alleviate this problem make sure of the following: the smaller you make the crop the more blurry the image, but higher resolution images can cope better with this. If you've uploaded images to your website yet when viewing them in Preview, or in Live mode, they appear blurry it is likely to be due to a combination of the original picture quality and the size of the crop you have set i.e.
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